山东及周边部分地区猪繁殖与呼吸综合征病毒的变异与遗传演化分析

doi:10.11843/j.issn.0366-6964.2019.06.015

Abstract

Abstract: In order to investigate the molecular epidemiological characteristics and genomic changes of porcine reproductive and respiratory syndrome virus (PRRSV) epidemic strains from Shandong province and its neighboring areas in China since 2017, in this study, 70 samples of suspected PRRS symptoms were collected from some pig farms in Shandong, Henan and Jiangsu provinces, and PRRSV was detected by RT-PCR. The genetic recombination of virus in PRRSV positive samples, the 561st, 586th and 592nd amino acids of Nsp9 which determine the pathogenicity and replication of Chinese highly pathogenic PRRSV (HP-PRRSV) were analyzed to explore the genetic evolution of PRRSV isolates. The results showed that 11 strains of PRRSV isolates were screened from the above samples, and the detection rate of PRRSV was 55.7%. Nsp2 sequence analysis showed that compared with the classical American strain VR-2332, 30 amino acids were discontinuously deleted at 481 and 533-561 sites in seven isolates, which were identical to the deletion characteristics of HP-PRRSV prevalent in China since 2006. Two PRRSV isolates had total deletions of 33 discontinuous amino acids in three fragments at 481, 533-561 and 595-597, respectively; and two PRRSV isolates showed a total of 73 discontinuous amino acid deletions in two fragments at 475-518 and 533-561, respectively. The Nsp2 genes of PRRSV mutant strains have undergone significant new deletions. The results of gene recombination analysis by the software RDP4 showed that the last four new type of deleted virus strains had large gene recombination, and the number of recombinant sites and recombinant fragments were different. The four strains all used the highly pathogenic strain JXA1 as the main parental strain for the recombinant and most of the recombination changes were concentrated on Nsp2 protein region, and partial recombination changes were also observed in the non-structural and minor protein regions. In addition, the amino acid variation analysis of positions 561, 586 and 592 of Nsp9 showed that the three critical amino acid sites of the four virus isolates were consistent with Chinese HP-PRRSV. This study accumulated data for further exploration of the genetic variation of PRRSV and related biological characteristics.

CLC Number:

S852.659.6

HU Dong, XU Yulin, ZHU Yingchun, ZHAO Qing, WANG Tingting, PANG Heng, LI Chuangang, YU Jiang, CHANG Weishan, WU Jiaqiang, PENG Jun. The Variations and Analyses in Genetic Evolution of Porcine Reproductive and Respiratory Syndrome Virus in Shandong Province and Its Neighboring Areas[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(6): 1249-1260.

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The Variations and Analyses in Genetic Evolution of Porcine Reproductive and Respiratory Syndrome Virus in Shandong Province and Its Neighboring Areas

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